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david chan  (Addgene inc)


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    Structured Review

    Addgene inc david chan
    David Chan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28/pET28-Mff(1-61)-PP-GST+(Plasmid+%2373042)/pmc12817251-589-7-9
    Average 94 stars, based on 4 article reviews
    david chan - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Synthesized:

    Article Title: Evaluating microbial protein elicitors for managing Cabbage Aphids in Brassica oleracea
    Article Snippet: Cabbage (Brassica oleracea) is a globally important crop but often suffers from damage caused by sucking insect pests and various diseases.. This study explores the effectiveness of microbial protein elicitors, such as PeaT1, PeBL1, Hrip1, and PeBA1, in managing Cabbage aphids (Brevicoryne brassicae).. It investigates how these elicitors affect phytohormone pathways, including jasmonic acid, salicylic acid, and ethylene, and examines their impact on leaf structures, particularly trichomes.

    Recombinant:

    Article Title: Evaluating microbial protein elicitors for managing Cabbage Aphids in Brassica oleracea
    Article Snippet: Cabbage (Brassica oleracea) is a globally important crop but often suffers from damage caused by sucking insect pests and various diseases.. This study explores the effectiveness of microbial protein elicitors, such as PeaT1, PeBL1, Hrip1, and PeBA1, in managing Cabbage aphids (Brevicoryne brassicae).. It investigates how these elicitors affect phytohormone pathways, including jasmonic acid, salicylic acid, and ethylene, and examines their impact on leaf structures, particularly trichomes.

    Article Title: PDZ-directed substrate recruitment is the primary determinant of specific 4E-BP1 dephosphorylation by PP1-Neurabin
    Article Snippet: Recombinant DNA reagent (plasmid) , pGEX 6P2 , GE Healthcare 27-4598-01 , , . .. Recombinant DNA reagent (plasmid) , pET28 PP1(7–300) , Dr. Wolfgang Peti , RRID: Addgene_26566 , . .. Recombinant DNA reagent (plasmid) , pET28 PP1-Phactr1 fusion , , , .

    other:

    Article Title: Highly specific intracellular ubiquitination of a small molecule.
    Article Snippet: Immunoblots were then imaged by using a Li-Cor Odyssey CLx imaging system with Image Studio 5.0 software.

    Clone Assay:

    Article Title: Highly specific intracellular ubiquitination of a small molecule
    Article Snippet: .. Briefly, HA–tUI protein cloned on pET28 (Addgene, 122662) was transformed into BL21 (DE3) Star II Escherichia coli (QB3-Berkeley) for protein expression. ..

    Article Title: Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
    Article Snippet: .. The gene coding for the Msm protein σ A was cloned into the pET28–MBP–TEV vector (a gift from Zita Balklava & Thomas Wassmer; Addgene plasmid #69929; http://n2t.net/addgene:69929 ) by the method of Restriction Free PCR cloning . ..

    Transformation Assay:

    Article Title: Highly specific intracellular ubiquitination of a small molecule
    Article Snippet: .. Briefly, HA–tUI protein cloned on pET28 (Addgene, 122662) was transformed into BL21 (DE3) Star II Escherichia coli (QB3-Berkeley) for protein expression. ..

    Expressing:

    Article Title: Highly specific intracellular ubiquitination of a small molecule
    Article Snippet: .. Briefly, HA–tUI protein cloned on pET28 (Addgene, 122662) was transformed into BL21 (DE3) Star II Escherichia coli (QB3-Berkeley) for protein expression. ..

    Plasmid Preparation:

    Article Title: PDZ-directed substrate recruitment is the primary determinant of specific 4E-BP1 dephosphorylation by PP1-Neurabin
    Article Snippet: Recombinant DNA reagent (plasmid) , pGEX 6P2 , GE Healthcare 27-4598-01 , , . .. Recombinant DNA reagent (plasmid) , pET28 PP1(7–300) , Dr. Wolfgang Peti , RRID: Addgene_26566 , . .. Recombinant DNA reagent (plasmid) , pET28 PP1-Phactr1 fusion , , , .

    Article Title: Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
    Article Snippet: .. The gene coding for the Msm protein σ A was cloned into the pET28–MBP–TEV vector (a gift from Zita Balklava & Thomas Wassmer; Addgene plasmid #69929; http://n2t.net/addgene:69929 ) by the method of Restriction Free PCR cloning . ..

    Article Title: TOP-SECRETS enables Cas9 nucleases to discriminate SNVs outside of PAMs
    Article Snippet: To generate a modified pSECRETS-A with SpRY Cas9, the gene for SpRY Cas9 was PCR amplified from the pET-SpRY-NLS-6xHis (gbr2101) plasmid (Addgene #181743) and combined with a PCR’ed segment of pSECRETS-A (amplified with a different primer set; Supplementary Table 1) via HiFi Assembly. .. The pET28-SpRY-NLS-6xHis (gbr2101) was a gift from Benjamin Kleinstiver (Addgene plasmid #181743; http://n2t.net/addgene:181743 ; RRID: Addgene_181743). .. To clone the x-gRNA libraries into pSECRETS-B (Addgene Plasmid #196987), a low copy number plasmid containing a kanamycin resistance cassette, for SECRETS, x-gRNA libraries containing a single spacer sequence and randomized 8 nt sequences (‘NNNNNNNN’) along with the “off-target” sequence (the “healthy” SNV, along with the 34 nucleotides flanking on each side) were cloned and maintained as previously described.

    Polymerase Chain Reaction:

    Article Title: Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
    Article Snippet: .. The gene coding for the Msm protein σ A was cloned into the pET28–MBP–TEV vector (a gift from Zita Balklava & Thomas Wassmer; Addgene plasmid #69929; http://n2t.net/addgene:69929 ) by the method of Restriction Free PCR cloning . ..

    Cloning:

    Article Title: Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
    Article Snippet: .. The gene coding for the Msm protein σ A was cloned into the pET28–MBP–TEV vector (a gift from Zita Balklava & Thomas Wassmer; Addgene plasmid #69929; http://n2t.net/addgene:69929 ) by the method of Restriction Free PCR cloning . ..



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